Phylogenetic analysis of Colchicum (Colchicaceae) genus by molecular markers from nuclear and chloroplast genome

  • Abdurrahman Umut Tüyel Department of Biomedical Engineering, Beykent University, Istanbul, Turkey; Marmara University, Faculty of Engineering, Department of Bioengineering, Göztepe Campus, 34722, Istanbul, Turkey
  • Ezgi Çabuk Şahin Marmara University, Faculty of Science and Art, Department of Biology, 34722, Istanbul, Turkey
  • Funda Şentürk Akfirat Gebze Technical University, Faculty of Basic Sciences, Department of Molecular Biology and Genetics, 41400, Gebze, Kocaeli, Turkey
  • Yıldız Aydin Marmara University, Faculty of Science and Art, Department of Biology, 34722, Istanbul, Turkey
  • Ahu Altınkut Uncuoğlu Marmara University, Faculty of Engineering, Department of Bioengineering, Göztepe Campus, 34722, Istanbul, Turkey

Abstract

We evaluated the patterns of genetic variation of 16 Colchicum species, including 37 different genotypes, using RAPD marker and trnL–trnF chloroplast DNA sequence. A total of 861 polymorphic alleles through RAPDs showed a mean of 33.88 ± 3.80 alleles per primer, while mean major allele frequency was 0.067 ± 0.05. The sequence length of trnL–trnF ranged from 1022 bp to 1081 bp. The phylogenetic tree was constructed to understand the relationship between Colchicum species and the discrimination power of the nuclear and chloroplast genome for species. The results showed that trnL–trnF gene region grouped Colchicum species well in comparison with RAPD analysis. This data was also supported by haplotype network analysis, structure analysis and PCA (Principle Component Analysis). This study showed that there is a need for a characterization that contains more molecular and morphological methods to correctly distinguish Colchicum species.

Published
2020-06-23
How to Cite
TÜYEL, Abdurrahman Umut et al. Phylogenetic analysis of Colchicum (Colchicaceae) genus by molecular markers from nuclear and chloroplast genome. Genetics & Applications, [S.l.], v. 4, n. 1, p. 1-11, june 2020. ISSN 2566-431X. Available at: <http://www.genapp.ba/index.php/genapp/article/view/112>. Date accessed: 11 july 2020. doi: https://doi.org/10.31383/ga.vol4iss1pp1-11.
Section
Research Articles