Laboratory testing and screening for SARS-CoV-2: A review of current methods

  • Damir Marjanović Institute for Anthropological Research, Zagreb, Croatia; International Burch University, Sarajevo, Bosnia and Herzegovina
  • Dragan Primorac St. Catherine Specialty Hospital, 49210 Zabok/ 10000 Zagreb, Croatia; Eberly College of Science, The Pennsylvania State University, University Park, State College, 16802 PA, USA; The Henry C. Lee College of Criminal Justice and Forensic Sciences, University of New Haven, West Haven, 06516 CT, USA; University of Split, School of Medicine, 21000 Split, Croatia; University “Josip Juraj Strossmayer”, Osijek, Faculty of Medicine, 31000 Osijek, Croatia; University “Josip Juraj Strossmayer”, Osijek, Faculty of Dental Medicine and Health, 31000 Osijek, Croatia; University of Rijeka, Faculty of Medicine, 51000 Rijeka, Croatia; Medical School REGIOMED, 96 450 Coburg, Germany; University of Mostar, School of Medicine, 88000 Mostar, Bosnia and Herzegovina
  • Vid Matišić St. Catherine Specialty Hospital, 49210 Zabok/ 10000 Zagreb, Croatia
  • Vitorio Perić St. Catherine Specialty Hospital, 49210 Zabok/ 10000 Zagreb, Croatia
  • Vilim Molnar St. Catherine Specialty Hospital, 49210 Zabok/ 10000 Zagreb, Croatia
  • Renata Zadro St. Catherine Specialty Hospital, 49210 Zabok/ 10000 Zagreb, Croatia

Abstract

In a relatively short period of time new coronavirus disease (COVID-19) has become a global threat, both to human health and to the functioning of human society in general. This pandemic is certainly neither the first nor is it likely to be the last disease episode in human history. At the moment, it is still too early to make a reliable assessment of its total effect on human civilization, but it can already be stated that this disease, and its causative agent SARS-CoV-2 virus, have caused a strong scientific response all around the World. For the first time in this magnitude, it has united the resources of large scientific institutions and companies with the aim of finding solutions for fast and accurate virus detection procedures, effective and safe vaccine, reliable medical treatments, etc. It is astonishing that only a month has passed from the first officially detected case to the complete sequencing of the SARS-CoV-2 virus genome and the creation of the first detection systems based on RT-PCR method. After that, numerous scientific teams and companies worked together, or independently, to improve the detection methods. Their work included further optimization of PCR and other genetic approaches, as well as the development of detection methods based on the analysis of specific antibodies and viral antigens. The aim of this paper is to review the results that were achieved in this area so far, analyze the strategies currently used in the world and the region, and to predict future steps in the process of optimizing and improving methods for SARS CoV-2 detection in individual patients and the global human population.

Published
2020-12-24
How to Cite
MARJANOVIĆ, Damir et al. Laboratory testing and screening for SARS-CoV-2: A review of current methods. Genetics & Applications, [S.l.], v. 4, n. 2, p. 1-9, dec. 2020. ISSN 2566-431X. Available at: <http://www.genapp.ba/index.php/genapp/article/view/128>. Date accessed: 14 apr. 2021. doi: https://doi.org/10.31383/ga.vol4iss2pp1-9.
Section
Reviews