Effects of Silymarin on Blood Glucose Concentration, Hepatic Histopathological Changes and FOXA2 and FOXA3 Gene Expression in Streptozotocin-Induced Diabetic Male Wistar Rats

  • Sajad Nikkhah Department of Biology, Science Faculty, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
  • Rahman Jafari Hafshejan Department of Biology, Science Faculty, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
  • Farshid Gheibi Hajivar Department of Biology, Science Faculty, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
  • Khalil Khashei Department of Genetics, School of Medicine, University of Islamic Azad, Kazerun, Iran
  • Sara Afzali Division of Cytogenetic, Medical Genetic Laboratory of Dr. Keshavarz, Guilan, Iran

Abstract

Since the liver is among the primary organs susceptible to the effects of hyperglycaemia, diabetes mellitus (DM) could be a risk factor for the development and progression of liver damage. In present study, since no side-effects from the herbal medicine have been reported, the effect of silymarin on blood glucose concentration, hepatic histopathological changes and FOXA2 and FOXA3 gene expression, which are key genes in liver regeneration, was investigated. In this fundamental with experimental approach study, 40 male Wistar rats weighing 180-220 g were used. Rats were kept under the standard conditions of temperature of 20-22°C and humidity of 50% and consecutive 12-hour periods of light and darkness. Rats were randomly divided into five different groups (n=8 each), including healthy control rats, diabetic control rats, diabetic rats receiving silymarin (50, 100 and 150 mg/kg). Diabetes was induced by injecting streptozotocin (50 mg/kg B.W., i.p.). For 4 weeks silymarin groups received the drug once every three days through gavage and fasting blood glucose concentration measured once every 10 days. At the end of a month experiment, livers were harvested for hepatic histopathological and FOXA2 and FOXA3 gene expression changes analysis. In the diabetic rats treated with silymarin (50, 100 and 150 mg/kg), by comparison with the diabetic control group (p<0.05), glucose levels decreased significantly. Moreover, FOXA2 and FOXA3 expression in diabetic groups treated with silymarin significantly increased compared to diabetic control group (p<0.05). Hepatic histopathological changes were improved in the treated groups.The present study indicates that silymarin significantly decreased blood glucose concentration and increased the FOXA2 and FOXA3 gene products level. Hence, silymarin is able to improve some of the symptoms associated with diabetes and possesses hepatoprotective effects in streptozotocin-induced diabetic rats.

Published
2021-12-27
How to Cite
NIKKHAH, Sajad et al. Effects of Silymarin on Blood Glucose Concentration, Hepatic Histopathological Changes and FOXA2 and FOXA3 Gene Expression in Streptozotocin-Induced Diabetic Male Wistar Rats. Genetics & Applications, [S.l.], v. 5, n. 2, p. 17-23, dec. 2021. ISSN 2566-431X. Available at: <http://www.genapp.ba/index.php/genapp/article/view/138>. Date accessed: 25 jan. 2022. doi: https://doi.org/10.31383/ga.vol5iss2pp17-23.
Section
Research Articles