The Applicability of Three DNA Isolation Methods in SSR Analysis of Hexaploid Plum (Prunus domestica L.) Cultivars

  • Jasna Hanjalić University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Bosnia and Herzegovina
  • Lejla Lasić University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Bosnia and Herzegovina
  • Fuad Gaši University of Sarajevo, Faculty of Agriculture, Sarajevo, Bosnia and Herzegovina
  • Mekjell Meland Norwegian Institute of Bioeconomy Research, Ǻs, Norway
  • Jasmin Grahić University of Sarajevo, Faculty of Agriculture, Sarajevo, Bosnia and Herzegovina
  • Belma Kalamujić Stroil University of Sarajevo-Institute for Genetic Engineering and Biotechnology http://orcid.org/0000-0002-4539-1266

Abstract

The main goal of any DNA extraction procedure is to ensure reliable and reproducible results in a simple, fast and inexpensive manner. When it comes to plant tissues, this goal is challenging to achieve due to the presence of a variety of metabolites that interfere with DNA during isolation and downstream analysis. In this study, we compared the efficiency of three methods for DNA extraction from plum kernels: 1) the standard CTAB Soltis method which is the most common protocol for DNA extraction from various plant tissues (seeds, young leafs, mature leafs, root); 2) CTAB-based method originally described for DNA isolation from medicinal plants with high levels of secondary metabolites; 3) and one of various commercially available kits. The usefulness of the obtained DNA was evaluated by SSR analysis with seven microsatellite markers. Although the latter two extraction protocols retrieved genomic DNA that gave positive PCR results, only DNA isolated by kit produced full SSR profile

Published
2018-06-21
How to Cite
HANJALIĆ, Jasna et al. The Applicability of Three DNA Isolation Methods in SSR Analysis of Hexaploid Plum (Prunus domestica L.) Cultivars. Genetics & Applications, [S.l.], v. 2, n. 1, p. 1-7, june 2018. ISSN 2566-431X. Available at: <http://www.genapp.ba/index.php/genapp/article/view/41>. Date accessed: 21 oct. 2018. doi: https://doi.org/10.31383/ga.vol2iss1pp1-7.
Section
Research Articles