Evaluation of Cytotoxicity and Genotoxicity of Micromeria pulegium (Rochel) Benth Extract in Human Lymphocytes and Gr-M Melanoma Cells in vitro

  • Anja Haverić University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Bosnia and Herzegovina
  • Jasmina Čakar University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Bosnia and Herzegovina
  • Maida Hadžić University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Bosnia and Herzegovina
  • Sanin Haverić University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Bosnia and Herzegovina

Abstract

Micromeria pulegium (Rochel) Benth. is an endemic species of Lamiacea family that includes frequently used plants in culinary and folk medicine. As cytotoxic potential of some species of Micromeria genus has been confirmed, this study aimed to test unknown antiproliferative and genotoxic potential of M. pulegium, endemic bh species, aqueous leaf extract in normal (human lymphocytes) and cancer (human melanoma GR-M) cells in order to protect small populations of native M. pulegium populations or promote its controlled micropropagation or cultivation. Cytokinesis-block micronucleus cytome assay was applied for human lymphocyte cultures, while trypan blue exclusion assay was used for evaluation of cytotoxicity in human GR-M melanoma cells. Results demonstrate no genotoxic effects up to concentration of 0.2 mg/ml in human lymphocyte in vitro but significant reduction of cell viability in human GR-M melanoma cell line cultures treated with 0.3 mg/ml of Micromeria extract.

Published
2018-06-21
How to Cite
HAVERIĆ, Anja et al. Evaluation of Cytotoxicity and Genotoxicity of Micromeria pulegium (Rochel) Benth Extract in Human Lymphocytes and Gr-M Melanoma Cells in vitro. Genetics & Applications, [S.l.], v. 2, n. 1, p. 25-31, june 2018. ISSN 2566-431X. Available at: <http://www.genapp.ba/index.php/genapp/article/view/43>. Date accessed: 17 dec. 2018. doi: https://doi.org/10.31383/ga.vol2iss1pp25-31.
Section
Research Articles