Frequency of ADH1B RsaI (rs2066701) single nucleotide polymorphism in a population of Bosnia and Herzegovina

  • Amar Pecikoza Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
  • Lejla Lasic University of Sarajevo - Institute for genetic engineering and biotechnology
  • Gabrijela Radosavljević University of Sarajevo - Institute for Genetic Engineering and Biotechnology
  • Anesa Ahatović University of Sarajevo - Institute for Genetic Engineering and Biotechnology
  • Ajla Smajlović Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
  • Adaleta Durmić-Pašić University of Sarajevo - Institute for Genetic Engineering and Biotechnology
  • Lejla Pojskić University of Sarajevo - Institute for Genetic Engineering and Biotechnology
  • Naris Pojskic University of Sarajevo - Institute for Genetic Engineering and Biotechnology

Abstract

Apart from its physiological role in the cellular oxidation of ethanol interesting feature of the ADH1B gene locus is its characteristic geographical distribution in which certain variants of ADH1B peak in different parts of the world.  Therefore, ADH1B rs2066701 polymorphism is exploited as a genetic marker in tracing of the evolutionary processes and human migrations in the past. Taking into consideration the complexity of population genetic structure and several migrations in the history of the Balkan populations, including Bosnian and Herzegovinian, this study aimed to estimate the frequency of ADH1B rs2066701 polymorphism in the population of Bosnia and Herzegovina. The total of 101 randomly sampled individuals was genotyped for rs2066701 polymorphism in ADH1B gene using PCR-RFLP method. The obtained frequencies were used to calculate heterozygosity, fixation indices and Hardy-Weinberg equilibrium. Observed population-structure parameters were compared with other population values available in ALFRED database. Dimensional relations between the investigated populations were visualised with the NM-MDS (non metric multidimensional scaling) analysis using PAST. The minor allele frequency for rs2066701 was 0,257. Inter-population analysis including other European and non-European populations from the ALFRED database proved the above-mentioned European genetic background of the B&H population.

Published
2018-12-25
How to Cite
PECIKOZA, Amar et al. Frequency of ADH1B RsaI (rs2066701) single nucleotide polymorphism in a population of Bosnia and Herzegovina. Genetics & Applications, [S.l.], v. 2, n. 2, p. 28-34, dec. 2018. ISSN 2566-431X. Available at: <http://www.genapp.ba/index.php/genapp/article/view/61>. Date accessed: 18 june 2019. doi: https://doi.org/10.31383/ga.vol2iss2pp28-34.
Section
Research Articles