Investigation of PTGS2, MAGE-A3, CALR, KRT19 and TMPRSS4 expressions in HCT116 colon cancer and PC3 prostate cancer cell lines

  • Süreyya Bozkurt Istinye University, Faculty of Medicine, Department of Medical Biology, Istanbul, Turkey
  • Filiz Yarimcan Medipol University, Faculty of Medicine, Department of Medical Microbiology, Istanbul, Turkey
  • Hüseyin Ayhan Istinye University, Faculty of Medicine, Department of Medical Biology, Istanbul, Turkey
  • Hacer Kotan Istinye University, Institute of Health Sciences, Department of Medical Biology and Genetics, Istanbul, Turkey
  • Hüma Tuğçe Sezgin Istinye University, Institute of Health Sciences, Department of Medical Biology and Genetics, Istanbul, Turkey
  • Elif Çınar Istinye University, Institute of Health Sciences, Department of Medical Biology and Genetics, Istanbul, Turkey
  • Ceren Aynacı Istinye University, Institute of Health Sciences, Department of Medical Biology and Genetics, Istanbul, Turkey
  • Remzi Okan Akar Istinye University, Department of Cancer Biology and Pharmacology, Institute of Medical Sciences, İstanbul, Turkey
  • Veysel Sabri Hançer Istinye University, Faculty of Medicine, Department of Medical Biology, Istanbul, Turkey

Abstract

Cancer is a disease arising from DNA alterations that dysregulate gene structure and function. These deregulated genes can also play a role in tumor invasion and metastasis or resistance to treatment. In this study, we determined the gene expression during transcription of PTGS2 (Prostaglandin-endoperoxide synthase 2), MAGE-A3 (Melanoma-associated antigen 3), CALR (Calreticulin), KRT19 (Cytokeratin 19), and TMPRSS4 (Transmembrane protease, serine 4) in HCT116 colon cancer cell line and PC3 prostate cancer cell line. After RNA isolation and cDNA conversion, DNA amplification was performed with Real-Time PCR. We determined the altered transcriptional expression level of those genes. In HCT116 colon cancer cell line, expression of the TMPRSS4 gene, MAGEA3 gene and KRT19 gene was found as increased and expression of the CALR gene and the PTGS2 gene was found as decreased. Especially a 93.70-fold increase in expression of the KRT19 gene was found in HCT116 colon cancer cell line. In PC3 prostate cancer cell lines, TMPRS4 gene expression and MAGEA3 gene expression were found as increased. But there was 50 fold decrease in PTGS2 gene expression.

Published
2020-12-24
How to Cite
BOZKURT, Süreyya et al. Investigation of PTGS2, MAGE-A3, CALR, KRT19 and TMPRSS4 expressions in HCT116 colon cancer and PC3 prostate cancer cell lines. Genetics & Applications, [S.l.], v. 4, n. 2, p. 37-42, dec. 2020. ISSN 2566-431X. Available at: <https://www.genapp.ba/index.php/genapp/article/view/120>. Date accessed: 12 june 2021. doi: https://doi.org/10.31383/ga.vol4iss2pp37-42.
Section
Research Articles