Cytotoxicity, Apoptosis induction and change of p53, PARP, p21 and Bcl-2 genes expression in the human anaplastic thyroid carcinoma cells line (SW-1736) with curcumin

Effects of curcumin on SW-1736 cells line

  • Khalil Khashei Department of Genetics, School of Medicine, University of Islamic Azad, Kazerun, Iran
  • Parinaz Tavakoli Department of Genetic, Shahrekord Branch, University of Islamic Azad, Shahrekord, Iran
  • Leila Rouhi Department of Physiology, Shahrekord Branch, University of Islamic Azad, Shahrekord, Iran
  • Somayee Raisi Department of Genetic, Shahrekord Branch, University of Islamic Azad, Shahrekord, Iran; Department of Genetic, Shahrekord University, Shahrekord, Iran

Abstract

Anaplastic thyroid carcinoma is highly invasive with a poor response to a treatment. In this study, curcumin bioavailability and its effects on apoptosis induction and selected genes expression of the human anaplastic thyroid carcinoma cell line (SW-1736) were examined. SW-1736 cells were incubated for 24 and 48 hours with different concentrations of curcumin 2.5, 5, 7.5 and 10 μM to examine bioavailability, and for 24, 48 and 72 hours with concentrations of 2.5, 5, 7.5, 10 μM and 2.5, 5 and 10 μM respectively to examine apoptosis and the expression of p53, PARP, p21 and Bcl-2 genes. Then, bioavailability was analyzed by MTS kit, apoptosis was analyzed by flow- cytometry using Annexin V-FITC/PI kit and the expression of p53, PARP, p21 and Bcl-2 genes were analyzed by Real Time PCR. ANOVA test and SPSS 16 software were used for statistical analysis. The results indicate that curcumin at the concentration of 7.5 μM has significantly decreased bioavailability in anaplastic thyroid cells in comparison with other treatments at both incubation periods. Induction of apoptosis with increasing concentration of curcumin in dose and time dependent manner increased in this cell line. Also, treatment with curcumin significantly decreased the expression of Bcl-2 gene and increased the expression of p53, PARP and p21 genes in some experimental groups compared to the control group. Curcumin inhibited the growth, proliferation and invasion of anaplastic thyroid cancer cells through altering the expression of the genes involved in the apoptosis process

Published
2021-06-21
How to Cite
KHASHEI, Khalil et al. Cytotoxicity, Apoptosis induction and change of p53, PARP, p21 and Bcl-2 genes expression in the human anaplastic thyroid carcinoma cells line (SW-1736) with curcumin. Genetics & Applications, [S.l.], v. 5, n. 1, p. 10-17, june 2021. ISSN 2566-431X. Available at: <https://www.genapp.ba/index.php/genapp/article/view/133>. Date accessed: 28 july 2021. doi: https://doi.org/10.31383/ga.vol5iss1pp10-17.
Section
Research Articles